The Mouse Apo-E (Apolipoprotein E) CLIA Kit is specifically designed for the accurate and precise measurement of Apo-E levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Apo-E is a critical protein involved in lipid metabolism and cardiovascular health, playing a crucial role in the regulation of cholesterol levels and the transport of lipids in the body.
Dysregulation of Apo-E has been associated with various diseases, including atherosclerosis, Alzheimer's disease, and other neurodegenerative disorders, making it a valuable biomarker for the study of these conditions and the development of potential therapeutic interventions.Overall, the Mouse Apo-E CLIA Kit provides researchers with a powerful tool for investigating the role of Apo-E in disease pathogenesis and evaluating potential treatment strategies in preclinical studies.
Product Name:
Mouse APO-E (Apolipoprotein E) CLIA Kit
SKU:
MOES00087
Target:
Mouse APO-E (Apolipoprotein E)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse APO-E. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse APO-E and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse APO-E, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse APO-E. You can calculate the concentration of Mouse APO-E in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
91-103
87-101
Average (%)
103
97
94
1:4
Range (%)
88-101
97-108
92-105
Average (%)
94
102
99
1:8
Range (%)
97-112
88-102
101-119
Average (%)
104
95
109
1:16
Range (%)
87-100
84-95
89-100
Average (%)
93
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
106
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
3.21
8.72
1.0
3.53
9.37
Standard deviation
0.1
0.37
0.99
0.1
0.41
0.86
C V (%)
9.8
11.53
11.35
10.0
11.61
9.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse APO-E concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse APO-E in samples. No significant cross-reactivity or interference between Mouse APO-E and analogues was observed.
