The Mouse APRIL (TNFSF13) ELISA Kit is a powerful tool for measuring levels of A Proliferation-Inducing Ligand (APRIL), also known as TNF- and APOL-related leukocyte expressed ligand 2 (TALL-2), in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it ideal for a variety of research applications. APRIL is a member of the tumor necrosis factor (TNF) superfamily and plays a crucial role in immune responses and inflammatory processes. It is involved in B cell survival, proliferation, and differentiation, making it a key player in humoral immunity.
Dysregulation of APRIL has been linked to autoimmune diseases, B cell malignancies, and other immune-related disorders, highlighting its importance as a biomarker for studying these conditions and developing potential therapeutic interventions. With the Mouse APRIL (TNFSF13) ELISA Kit, researchers can delve deeper into the intricate mechanisms of immune regulation and pathology, advancing our understanding of how APRIL influences disease progression and potentially uncovering new treatment strategies. Get reliable and insightful results with this advanced ELISA kit from Assay Genie.
Product Name:
Mouse APRIL/TNFSF13 ELISA Kit
SKU:
MOFI00483
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.375 ng/mL
Range:
0.625-40 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-104%
82-93%
81-95%
EDTA Plasma (n = 5)
85-103%
89-97%
80-93%
Heparin Plasma (n = 5)
86-100%
85-101%
80-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-104
95
EDTA Plasma (n = 5)
86-95
91
Heparin Plasma (n = 5)
88-102
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
