The Mouse Beta-EP (Beta-Endorphin) ELISA Kit is specifically designed for the accurate quantification of beta-endorphin levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications. Beta-endorphin is a key neuropeptide involved in pain modulation, stress response, and mood regulation. It plays a critical role in various physiological processes and has been implicated in conditions such as chronic pain, addiction, and mood disorders. By measuring beta-endorphin levels, researchers can gain valuable insights into these complex mechanisms and potential therapeutic interventions.
Product Name:
Mouse beta-EP (Beta-Endorphin) ELISA Kit
SKU:
MOES00767
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
