The Rat β-EP (Beta-Endorphin) ELISA Kit is meticulously designed to quantitatively assess the levels of Beta-Endorphin in diverse rat biological samples. Beta-Endorphin, a prominent member of the endogenous opioid peptides, holds significant importance in regulating pain perception and the body's stress response. As a natural opioid with potent analgesic properties, Beta-Endorphin plays a vital role in modulating mood, stress, and emotions. Understanding the dynamics of Beta-Endorphin levels is crucial for unraveling its impact on neurological functions, pain management, and emotional well-being. This ELISA kit is tailored to deliver exceptional sensitivity and specificity, ensuring precise and reproducible results in research applications. Manufactured under strict quality control standards, the Rat β-EP ELISA Kit offers robust performance, making it a reliable choice for researchers exploring the intricate interplay of Beta-Endorphin in various physiological processes. Delve into the realm of opioid peptides and pain modulation with confidence using this advanced ELISA kit, unlocking insights into Beta-Endorphin's role in neurobiology and behavioral responses.
Product Name:
Rat beta-EP (Beta-Endorphin) ELISA Kit
SKU:
AEES00324
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
