Mouse BMI-1 (Polycomb complex protein BMI-1) ELISA Kit
The Mouse BMI-1 Polycomb Complex Protein BMI-1 ELISA Kit is a valuable tool for researchers looking to quantify levels of BMI-1 in mouse samples with high sensitivity and accuracy. This kit is designed for use with mouse serum, plasma, and cell culture supernatants, providing reliable and reproducible results for a variety of experimental applications. BMI-1 is a key component of the polycomb repressive complex 1 (PRC1), playing a critical role in the regulation of gene expression and cell proliferation. Dysregulation of BMI-1 has been linked to various diseases, including cancer and neurological disorders, making it an important marker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and optimized protocols, the Mouse BMI-1 Polycomb Complex Protein BMI-1 ELISA Kit offers researchers a powerful tool for investigating the role of BMI-1 in disease progression and developing targeted treatments. Trust Assay Genie for high-quality ELISA kits that deliver accurate and reliable results for your research needs.
Product Name:
Mouse BMI-1 (Polycomb complex protein BMI-1) ELISA Kit
SKU:
MOFI01238
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
4.688 pg/mL
Range:
7.813-500 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
92-105%
89-98%
80-100%
EDTA Plasma (n = 5)
85-100%
87-101%
85-98%
Heparin Plasma (n = 5)
91-105%
82-95%
82-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-104
95
EDTA Plasma (n = 5)
86-102
94
Heparin Plasma (n = 5)
95-101
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
