The Mouse Carnosine Synthase 1 (CarnS1) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of CarnS1 in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers precise and consistent results, making it an invaluable asset for a variety of experimental studies.CarnS1 is an enzyme crucial in the biosynthesis of carnosine, a dipeptide with numerous biological roles including antioxidant properties, anti-inflammatory effects, and potential anti-aging benefits. Dysregulation of carnosine metabolism has been linked to various diseases such as diabetes, neurodegenerative disorders, and muscle-related conditions, highlighting the importance of monitoring CarnS1 levels.
By utilizing the Mouse CarnS1 ELISA Kit, researchers can gain valuable insights into the role of carnosine in health and disease, paving the way for innovative therapies and interventions. Whether investigating basic biological mechanisms or exploring potential clinical applications, this kit provides the precision and reliability needed for cutting-edge research in the field of carnosine metabolism.
Product Name:
Mouse Carnosine Synthase 1 (CARNS1) ELISA Kit
Product Code:
MODL00202
Size:
96 Assays
Target:
CARNS1
Synonyms:
ATPGD1, ATP-Grasp Domain Containing 1
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
