The Mouse CNTN1 (Contactin-1) CLIA Kit is designed for the precise quantification of contactin-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Contactin-1 is a critical protein that plays a key role in cell adhesion and axon guidance, crucial processes in nervous system development and function. Dysregulation of contactin-1 has been associated with neurological disorders such as autism and multiple sclerosis, making it a valuable biomarker for understanding these conditions and potential therapeutic interventions.
The Mouse CNTN1 CLIA Kit from Assay Genie provides researchers with a reliable tool for studying the role of contactin-1 in neurological function and disease, enabling advancements in neurobiology research. Get your kit today and unlock new insights into the intricate mechanisms of the nervous system.
Product Name:
Mouse CNTN1 (Contactin 1) CLIA Kit
SKU:
MOES00199
Target:
Mouse CNTN1 (Contactin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CNTN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CNTN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CNTN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CNTN1. You can calculate the concentration of Mouse CNTN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
85-98
93-105
Average (%)
106
90
99
1:4
Range (%)
91-102
97-113
85-96
Average (%)
97
103
91
1:8
Range (%)
99-116
92-106
100-117
Average (%)
107
98
107
1:16
Range (%)
94-109
98-114
100-113
Average (%)
100
106
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-105
96
EDTA plasma (n=5)
98-114
106
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.76
255.73
795.17
99.45
256.21
762.6
Standard deviation
9.61
22.58
80.39
10.07
30.49
50.48
C V (%)
9.09
8.83
10.11
10.13
11.9
6.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CNTN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CNTN1 in samples. No significant cross-reactivity or interference between Mouse CNTN1 and analogues was observed.
