The Mouse DNMT1 (DNA Cytosine-5-methyltransferase 1) ELISA Kit is a valuable tool for the quantitative measurement of DNMT1 levels in mouse samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications. DNMT1 is a key enzyme responsible for maintaining DNA methylation patterns during cell division and plays a critical role in gene regulation and cellular differentiation. Dysregulation of DNMT1 has been implicated in various diseases, including cancer, neurological disorders, and developmental abnormalities, making it a vital target for investigation and therapeutic development.
By utilizing the Mouse DNMT1 ELISA Kit, researchers can effectively study the role of DNMT1 in disease pathogenesis, identify potential biomarkers for diagnostic and prognostic purposes, and evaluate the efficacy of DNMT1-targeted therapies. Trust in the precision and reliability of this ELISA kit to advance your research efforts in the field of epigenetics and molecular biology.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-105%
87-105%
85-93%
EDTA Plasma (n = 5)
88-100%
87-101%
82-96%
Heparin Plasma (n = 5)
80-100%
80-96%
88-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-99
91
EDTA Plasma (n = 5)
88-104
93
Heparin Plasma (n = 5)
96-105
100
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
