The Mouse Fe-Ferritin CLIA Kit is a cutting-edge assay designed for the precise measurement of mouse ferritin levels in biological samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Ferritin is a key protein involved in iron metabolism, playing a vital role in storing and releasing iron in the body. Abnormal ferritin levels have been linked to several health conditions including iron overload disorders, anemia, and inflammatory diseases. Therefore, monitoring ferritin levels can provide valuable insights into the underlying mechanisms of these diseases and aid in the development of potential treatments.With its advanced technology and user-friendly protocols, the Mouse Fe-Ferritin CLIA Kit from Assay Genie is a valuable tool for researchers studying iron metabolism and related disorders in mouse models. Get reliable and insightful data with this innovative assay kit.
Product Name:
Mouse FE (Ferritin) CLIA Kit
SKU:
MOES00257
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
