The Mouse Fibrinogen Alpha (FGA) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of Fibrinogen Alpha levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for various research applications in the field of coagulation and thrombosis.Fibrinogen Alpha is a key component of the blood clotting cascade, essential for forming fibrin clots to stop bleeding. Dysregulation of Fibrinogen Alpha can lead to various thrombotic disorders, including venous thrombosis and disseminated intravascular coagulation.
Monitoring Fibrinogen Alpha levels is crucial for understanding these conditions and developing targeted therapies.With its high sensitivity and specificity, the Mouse Fibrinogen Alpha (FGA) ELISA Kit is an invaluable tool for researchers studying coagulation disorders and thrombotic diseases in mouse models. Get accurate and reliable results with this innovative assay kit from AssayGenie.
Product Name:
Mouse Fibrinogen Alpha (FGa) ELISA Kit
Product Code:
MODL00469
Size:
96 Assays
Target:
FGa
Synonyms:
Fib2, FG-A, Fibrinopeptide A
Detection Method:
Sandwich
Range:
31.25-2000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
