The Mouse Fibulin-3 (FBLN3) ELISA Kit is a powerful tool for measuring fibulin-3 levels in mouse serum, plasma, and cell culture supernatants with high precision and accuracy. Fibulin-3 is an important extracellular matrix protein that plays a role in cell adhesion, migration, and signaling, making it a valuable target for investigating various biological processes.This ELISA kit offers superior sensitivity and specificity, ensuring dependable and reproducible results for a wide range of research applications. Fibulin-3 is implicated in diseases such as cancer, fibrosis, and cardiovascular disorders, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.
Researchers seeking to explore the functions of fibulin-3 and its implications in disease pathogenesis will find the Mouse Fibulin-3 ELISA Kit to be an indispensable tool for their experiments. With its easy-to-follow protocol and reliable performance, this kit is a valuable asset for investigating the role of fibulin-3 in physiological and pathological processes.
Product Name:
Mouse Fibulin 3 (FBLN3) ELISA Kit
Product Code:
MODL00460
Size:
96 Assays
Target:
FBLN3
Synonyms:
EFEMP1, DHRD, DRAD, FBNL, MLVT, MTLV, S1-5, EGF-Containing Fibulin-Like Extracellular Matrix Protein 1, Fibrillin-Like, Extracellular protein S1-5
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
