Mouse FK506 Binding Protein Like Protein (FKBPL) ELISA Kit
The Mouse FK506 Binding Protein-like Protein (FKBPL) ELISA Kit is a reliable and efficient tool for the precise measurement of FKBPL levels in various biological samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and reproducible results, making it suitable for a wide range of research applications.FKBPL is a protein known to be involved in various cellular processes, including protein folding and trafficking.
It has been implicated in different disease conditions, such as cancer and neurodegenerative disorders, making it a valuable biomarker for studying these diseases and potential therapeutic targets.This ELISA kit is an essential tool for researchers interested in uncovering the role of FKBPL in various biological processes and diseases, ultimately leading to a better understanding of its function and potential clinical applications.
Product Name:
Mouse FK506 Binding Protein Like Protein (FKBPL) ELISA Kit
Product Code:
MODL00492
Size:
96 Assays
Target:
FKBPL
Synonyms:
NG7, WISP39, DIR1, WAF-1/CIP1 stabilizing protein 39
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
