Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
The Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit is meticulously designed for the quantitative detection of GDNF in various mouse biological samples. GDNF, a potent neurotrophic factor, is critically involved in promoting the growth and survival of neurons, particularly dopaminergic neurons, and plays a key role in neuronal development and maintenance. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for researchers investigating the functions and therapeutic potential of GDNF in various neurological processes. Manufactured under strict quality control standards, this kit provides reliable performance, making it an excellent choice for neurobiology research and the study of neurotrophic factors.
Product Name:
Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
SKU:
AEES00223
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
