Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) CLIA Kit
The Mouse GDNF (Glial Cell line-Derived Neurotrophic Factor) CLIA Kit is a cutting-edge tool for precise measurement of GDNF levels in mouse serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for various research applications.GDNF is a pivotal factor in neuronal development and survival, particularly in the central nervous system.
Its role in promoting nerve growth and protecting dopaminergic neurons has made it a key target in the study of neurodegenerative diseases like Parkinson's and Huntington's. The Mouse GDNF CLIA Kit provides researchers with a valuable tool for investigating GDNF's functions and potential therapeutic applications in these conditions.
Product Name:
Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) CLIA Kit
SKU:
MOES00289
Target:
Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDNF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GDNF. You can calculate the concentration of Mouse GDNF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
102-119
85-96
Average (%)
90
109
91
1:4
Range (%)
87-100
94-109
102-115
Average (%)
94
99
109
1:8
Range (%)
94-106
93-106
94-107
Average (%)
100
99
99
1:16
Range (%)
89-101
97-112
95-108
Average (%)
95
103
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
103
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
93-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.1
114.36
448.12
58.01
115.89
468.58
Standard deviation
4.64
10.22
48.98
4.77
9.95
37.44
C V (%)
8.42
8.94
10.93
8.22
8.59
7.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDNF in samples. No significant cross-reactivity or interference between Mouse GDNF and analogues was observed.
