Rat GDNF (Glial Cell Line Derived Neurotrophic Factor) CLIA Kit
The Rat GDNF (Glial Cell Line-Derived Neurotrophic Factor) CLIA Kit is a state-of-the-art assay designed for the accurate quantification of GDNF levels in rat samples such as serum, plasma, and tissue lysates. This kit offers superior sensitivity and specificity, ensuring reliable and consistent results for your research needs.GDNF is a vital factor in the nervous system, promoting the survival and differentiation of neurons. It has implications in various neurological disorders, making it a crucial biomarker for studying conditions like Parkinson's disease, Alzheimer's disease, and spinal cord injuries.
With its advanced technology and user-friendly protocol, the Rat GDNF CLIA Kit is a valuable tool for researchers looking to delve deeper into the role of GDNF in neurobiology and potential therapeutic interventions. Get your hands on this innovative kit today and unlock new insights into neurotrophic factor research.
Product Name:
Rat GDNF (Glial Cell Line Derived Neurotrophic Factor) CLIA Kit
SKU:
RTES00238
Target:
Rat GDNF (Glial Cell Line Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDNF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GDNF. You can calculate the concentration of Rat GDNF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
92-106
102-115
Average (%)
97
98
108
1:4
Range (%)
92-102
88-102
92-105
Average (%)
97
94
98
1:8
Range (%)
97-113
91-101
98-110
Average (%)
104
96
104
1:16
Range (%)
93-111
98-112
92-104
Average (%)
101
106
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.07
121.99
408.12
54.44
128.92
436.94
Standard deviation
4.46
13.91
45.22
6.07
13.25
39.41
C V (%)
8.25
11.4
11.08
11.15
10.28
9.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GDNF in samples. No significant cross-reactivity or interference between Rat GDNF and analogues was observed.
