Mouse Glutathione S Transferase Omega 1 (GSTo1) ELISA Kit
The Mouse Glutathione S-Transferase Omega 1 (GSTO1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of GSTO1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying the role of GSTO1 in various biological processes.GSTO1 is an enzyme that plays a crucial role in cellular detoxification and antioxidant defense mechanisms. Dysregulation of GSTO1 has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, accurate measurement of GSTO1 levels is important for understanding its role in disease pathogenesis and developing potential therapeutic interventions.With its high sensitivity and specificity, the Mouse GSTO1 ELISA Kit from Assay Genie is a valuable tool for researchers looking to study the role of GSTO1 in mouse models and explore its potential as a therapeutic target. Order your kit today and unlock new insights into the biology of GSTO1.
Product Name:
Mouse Glutathione S Transferase Omega 1 (GSTo1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
90-102
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
