SynonymsF1,B-50,PP46,Neuron Growth-Associated Protein 43,Neuromodulin,Axonal Membrane Protein GAP-43,Protein F1,Calmodulin-Binding Protein P-57,Neural Phosphoprotein B-50
ReactivityMouse
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
MODL00527
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06837
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
F1,B-50,PP46,Neuron Growth-Associated Protein 43,Neuromodulin,Axonal Membrane Protein GAP-43,Protein F1,Calmodulin-Binding Protein P-57,Neural Phosphoprotein B-50
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Mouse Growth Associated Protein 43 (GAP43) ELISA Kit
The Mouse Growth-Associated Protein 43 (GAP43) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of GAP43 in mouse samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it a valuable tool for various research applications.GAP43 is a protein known for its role in neuronal development and plasticity, making it essential for processes such as axon growth and regeneration.
By accurately measuring GAP43 levels, researchers can gain valuable insights into neurological disorders, nerve injury, and synaptic plasticity, paving the way for potential therapeutic advancements.Overall, the Mouse GAP43 ELISA Kit is a crucial resource for researchers looking to study the role of GAP43 in neuroscience and neurology, offering accurate and precise measurements for impactful research outcomes.
Product Name:
Mouse Growth Associated Protein 43 (GAP43) ELISA Kit
Product Code:
MODL00527
Size:
96 Assays
Target:
GAP43
Synonyms:
F1, B-50, PP46, Neuron Growth-Associated Protein 43, Neuromodulin, Axonal Membrane Protein GAP-43, Protein F1, Calmodulin-Binding Protein P-57, Neural Phosphoprotein B-50
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
