Description
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Mouse Growth Factor Array (30 targets) (SARB0088)
The Mouse Growth Factor Array (SARB0088) from Assay Genie is a powerful tool for studying the expression levels of 30 different growth factors in mouse samples. This array allows for high-throughput analysis, saving time and resources compared to traditional methods.With targets including insulin-like growth factor, vascular endothelial growth factor, and fibroblast growth factor, researchers can gain valuable insights into the signalling pathways involved in growth and development. The array is easy to use and provides accurate and reliable results, making it ideal for a wide range of research applications in areas such as developmental biology, cancer research, and regenerative medicine.
By utilizing the Mouse Growth Factor Array from Assay Genie, researchers can accelerate their studies and uncover new findings related to growth factor signalling in mouse models. This comprehensive array is a valuable asset for any lab looking to expand their understanding of growth factor biology.
| Product Sku: | SARB0088 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 30 |
| Gene Symbols: | AREG, CSF1, CSF2, CSF3, EGF, EGFR, FGF2, FGF7, FIGF, FLT1|FLT4, HGF, IGF1, IGF1R, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL2, IL7, KDR, KITLG, MET, NGF, PDGFA, PDGFB, PGF, TGFB1, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | IGF Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Amphiregulin
| beta-NGF
| bFGF
| EGF
| EGFR
|
FGF-7 (KGF)
| GCSF
| GM-CSF
| HGF
| HGFR
|
IGF-1
| IGF-1 R
| IGF-2
| IGFBP-2
| IGFBP-3
|
IGFBP-5
| IGFBP-6
| IL-2
| IL-7
| M-CSF
|
PDGF-AA
| PDGF-BB
| PLGF-2
| SCF
| TGF beta 1
|
VEGF-A
| VEGF-D
| VEGFR1
| VEGFR2
| VEGFR3
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Growth Factor Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
