The Mouse IFN-Beta (Interferon Beta) CLIA Kit is specifically designed for the quantitative detection of Interferon Beta levels in mouse serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and reproducible results, making it perfect for a variety of research applications.Interferon Beta is a key cytokine involved in the immune response, playing a crucial role in antiviral defense and modulating inflammatory processes.
Aberrant levels of IFN-Beta have been implicated in autoimmune disorders, viral infections, and inflammatory diseases, highlighting its importance as a biomarker for studying these conditions and developing potential treatments.Overall, the Mouse IFN-Beta CLIA Kit offers researchers a reliable tool for studying the role of Interferon Beta in various biological processes, providing valuable insights into immune regulation and disease pathology.
Product Name:
Mouse IFN- beta (Interferon Beta) CLIA Kit
SKU:
MOES00030
Target:
Mouse IFN- beta (Interferon Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IFN-β. You can calculate the concentration of Mouse IFN-β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
99-111
100-114
Average (%)
103
105
106
1:4
Range (%)
92-107
96-112
87-98
Average (%)
98
102
92
1:8
Range (%)
93-105
93-104
97-112
Average (%)
98
98
103
1:16
Range (%)
99-114
95-107
96-111
Average (%)
107
101
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
96
EDTA plasma (n=5)
94-105
99
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.9
61.75
235.46
28.25
67.37
232.88
Standard deviation
2.72
6.18
19.59
2.63
5.89
26.18
C V (%)
10.5
10.01
8.32
9.31
8.74
11.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IFN-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IFN-β in samples. No significant cross-reactivity or interference between Mouse IFN-β and analogues was observed.
