The Mouse IgA (Immunoglobulin A) CLIA Kit is a powerful tool for quantifying IgA levels in mouse serum, plasma, and other biological samples. This kit offers exceptional sensitivity and accuracy, providing researchers with reliable and reproducible results for a variety of applications.Immunoglobulin A is a key component of the immune system, helping to protect the body against pathogens and infections.
Monitoring IgA levels can provide valuable insight into immune responses, inflammatory conditions, and autoimmune disorders in mice.With its ease of use and high-performance capabilities, the Mouse IgA CLIA Kit is an essential resource for studies in immunology, infectious diseases, and drug development. Order yours today and take your research to the next level.
Product Name:
Mouse IgA (Immunoglobulin A) CLIA Kit
SKU:
MOES00375
Target:
Mouse IgA (Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IgA. You can calculate the concentration of Mouse IgA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
99-115
91-108
Average (%)
95
105
99
1:4
Range (%)
102-117
98-116
91-103
Average (%)
109
106
96
1:8
Range (%)
101-113
96-111
88-99
Average (%)
107
104
93
1:16
Range (%)
90-105
101-117
104-119
Average (%)
97
107
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
100-115
106
Cell culture media (n=5)
99-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.9
11.08
27.73
3.56
10.16
27.83
Standard deviation
0.32
1.15
1.88
0.4
1.14
3.11
C V (%)
8.21
10.38
6.78
11.24
11.22
11.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgA in samples. No significant cross-reactivity or interference between Mouse IgA and analogues was observed.
