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Mouse IGF-2 PharmaGenie ELISA Kit (SBRS1399)

SKU:
SBRS1399
Product Type:
ELISA Kit
Reactivity:
Mouse
Applications:
ELISA
ELISA Type:
Sandwich
€635
Frequently bought together:

Description

system_update_altDatasheet

Mouse IGF-2 PharmaGenie ELISA Kit (SBRS1399)

Product SKU:SBRS1399
Size:96T
Application:The antibody pair provided in this kit recognizes mouse IGF-II (for Cell Culture).
Uniprot:P09535
Gene ID:16002
Gene Names:IGF-2 / IGF2
Synonyms:Insulin-like growth factor II (IGF-II) (Multiplication-stimulating polypeptide) [Cleaved into: Insulin-like growth factor II Preptin]
Target Species:Mouse
Compatible Sample Types:Cell Culture Supernatants
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Sensitivity:1500 pg/ml
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Stop Solution
  4. Assay Diluent(s)
  5. Lyophilized Standard
  6. Biotinylated Detection Antibody
  7. Streptavidin-Conjugated HRP
  8. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Mouse IGF-2 PharmaGenie ELISA Kit (SBRS1399) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.

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