The Mouse IgG (Immunoglobulin G) ELISA Kit, available at www.assaygenie.com/mouse-igg-immunoglobulin-g-elisa-kit-moes01189/, is a reliable and accurate tool for quantifying mouse IgG levels in various biological samples. With its high sensitivity and specificity, this kit delivers precise and reproducible results, making it ideal for a wide range of research applications.Immunoglobulin G, or IgG, is a key component of the immune system and plays a crucial role in immune responses against pathogens. By measuring IgG levels, researchers can gain valuable insights into the immune status and response of experimental animals. This ELISA kit offers a convenient and efficient way to study the humoral immune response in mouse models, aiding in the understanding of various diseases and conditions.Overall, the Mouse IgG ELISA Kit is a valuable tool for researchers working in immunology, infectious diseases, and translational medicine, providing accurate and reliable data for their studies.
Product Name:
Mouse IgG (Immunoglobulin G) ELISA Kit
SKU:
MOES01189
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
