The Mouse IgG2a (Immunoglobulin G2a) ELISA Kit is specifically designed for the accurate quantification of IgG2a levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it an excellent tool for various research applications.Immunoglobulin G2a is an important antibody subclass involved in immune responses, particularly in antibody-mediated immunity. Detection and measurement of IgG2a levels are essential for studying immune responses, monitoring disease progression, and evaluating potential treatments in various experimental models.Overall, the Mouse IgG2a ELISA Kit provides researchers with a reliable and efficient method for quantifying IgG2a levels in mouse samples, facilitating a better understanding of immune responses and immunological processes in research studies.
Product Name:
Mouse IgG2a (Immunoglobulin G2a) ELISA Kit
SKU:
MOES01191
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
