The Mouse Lactoferrin (LTF) ELISA Kit is specifically designed for the precise measurement of lactoferrin levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and accuracy, guaranteeing consistent and dependable results for various research purposes.Lactoferrin is a multifunctional protein known for its role in immune regulation, antimicrobial activity, and iron binding. It plays a crucial role in promoting gut health, supporting the immune system, and providing protection against infections and inflammation.
Monitoring lactoferrin levels in biological samples can provide valuable insights into various physiological and pathological processes, making it an invaluable tool for researchers in immunology, microbiology, and nutrition.Overall, the Mouse Lactoferrin (LTF) ELISA Kit is a valuable asset for investigating the functions and mechanisms of lactoferrin in mouse models, offering researchers a reliable and efficient method for detecting and quantifying lactoferrin levels in biological samples.
Product Name:
Mouse Lactoferrin (LTF) ELISA Kit
Product Code:
MODL00831
Size:
96 Assays
Target:
LTF
Synonyms:
HLF2, LF, GIG12, Lactotransferrin, Growth-inhibiting protein 12, Talalactoferrin, Lfcin-H, Kaliocin-1
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
