The Mouse LTF (LF Lactoferrin) ELISA Kit is specifically designed for the precise measurement of LF Lactoferrin levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring dependable and consistent results, thereby making it an excellent choice for a variety of research applications.LF Lactoferrin is a key protein known for its antimicrobial and immunomodulatory properties. It plays a crucial role in the immune response, inflammation, and iron metabolism, making it a valuable biomarker for studying various health conditions and potential therapeutic avenues.With easy-to-follow protocols and rapid assay procedures, the Mouse LTF (LF Lactoferrin) ELISA Kit provides researchers with a highly efficient tool for investigating the role of LF Lactoferrin in different biological processes and diseases.
Product Name:
Mouse LTF/LF (Lactoferrin) ELISA Kit
SKU:
MOES01235
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
