The Mouse LEP (Leptin) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of leptin levels in mouse serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Leptin is a key hormone that regulates appetite and energy balance, playing a crucial role in metabolism and weight regulation. Dysregulation of leptin levels has been associated with obesity, diabetes, and other metabolic disorders, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.
With its high sensitivity and precision, the Mouse LEP CLIA Kit offers a powerful tool for researchers looking to investigate the role of leptin in physiological and pathological processes in mouse models.
Product Name:
Mouse LEP (Leptin) CLIA Kit
SKU:
MOES00036
Target:
Mouse LEP (Leptin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-118
91-106
99-115
Average (%)
109
98
107
1:4
Range (%)
101-115
88-103
85-97
Average (%)
107
95
90
1:8
Range (%)
100-115
87-101
90-103
Average (%)
108
93
97
1:16
Range (%)
100-114
88-101
97-113
Average (%)
106
93
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.04
113.36
422.1
45.66
118.87
414.18
Standard deviation
5.83
12.57
30.69
5.72
9.13
34.21
C V (%)
12.39
11.09
7.27
12.53
7.68
8.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse LEP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LEP in samples. No significant cross-reactivity or interference between Mouse LEP and analogues was observed.
