SynonymsTBM,MUC5,LeB,Oligomeric Mucus/Gel-Forming,Mucin 5,Subtypes A And C,Tracheobronchial/Gastric,Gastric mucin,Lewis B blood group antigen,Major airway glycoprotein
ReactivityMouse
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
MODL00904
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
E9PWB6
Sensitivity:
0.137ng/mLL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
TBM,MUC5,LeB,Oligomeric Mucus/Gel-Forming,Mucin 5,Subtypes A And C,Tracheobronchial/Gastric,Gastric mucin,Lewis B blood group antigen,Major airway glycoprotein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Mouse Mucin 5 Subtype AC (MUC5AC) ELISA Kit is specially designed for the precise measurement of MUC5AC levels in mouse serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it the perfect tool for various research applications.MUC5AC is a key mucin protein that is essential for maintaining normal mucosal function in the respiratory and gastrointestinal tracts. Aberrant expression of MUC5AC has been linked to various respiratory diseases, such as asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Mouse MUC5AC ELISA Kit provides researchers with a reliable and efficient method for quantifying MUC5AC levels in mouse samples, advancing our understanding of mucin biology and its implications in disease pathology.
Product Name:
Mouse Mucin 5 Subtype AC (MUC5AC) ELISA Kit
Product Code:
MODL00904
Size:
96 Assays
Target:
MUC5AC
Synonyms:
TBM, MUC5, LeB, Oligomeric Mucus/Gel-Forming, Mucin 5,Subtypes A And C,Tracheobronchial/Gastric, Gastric mucin, Lewis B blood group antigen, Major airway glycoprotein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
