The Mouse Multimerin-2 (Mmrn2) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of Multimerin-2 levels in mouse serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it an ideal tool for a wide range of research applications.Multimerin-2 is a key protein involved in various biological processes, including platelet adhesion and aggregation, as well as vascular development. It plays a crucial role in maintaining vascular integrity and clotting functions, making it a valuable biomarker for studying cardiovascular diseases, thrombosis, and other related disorders.
With its high specificity and sensitivity, the Mouse Multimerin-2 (Mmrn2) ELISA Kit offers researchers a reliable and efficient method for measuring Multimerin-2 levels in mouse samples, providing valuable insight into the role of this protein in physiological and pathological conditions.
Product Name:
Mouse Multimerin 2 (MMRN2) ELISA Kit
Product Code:
MODL00883
Size:
96 Assays
Target:
MMRN2
Synonyms:
EMILIN3, EndoGlyx-1, Elastin Microfibril Interfacer 3, Elastin microfibril interface located protein 3, EndoGlyx-1 p125/p140 subunit
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
