The Mouse Myo (Myoglobin) CLIA Kit is specifically designed for the precise measurement of myoglobin levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it perfect for various research studies.Myoglobin is a key protein found in muscle tissue, playing a vital role in oxygen storage and utilization. Elevated levels of myoglobin can indicate muscle damage or injury, making it a valuable marker for studying conditions such as muscular dystrophy, myocardial infarction, and exercise-induced muscle damage.
With this Mouse Myo (Myoglobin) CLIA Kit, researchers can easily quantify myoglobin levels in mouse samples, allowing for a better understanding of muscle function and pathology. Its user-friendly protocol and reliable performance make it an essential tool for any laboratory studying muscle-related diseases and conditions.
Product Name:
Mouse MYO (Myoglobin) CLIA Kit
SKU:
MOES00064
Target:
Mouse MYO (Myoglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MYO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MYO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MYO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MYO. You can calculate the concentration of Mouse MYO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
91-103
97-108
Average (%)
104
96
102
1:4
Range (%)
91-105
87-100
93-106
Average (%)
99
93
99
1:8
Range (%)
86-100
87-101
83-98
Average (%)
91
94
90
1:16
Range (%)
102-115
96-110
87-100
Average (%)
109
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.0
2.47
7.3
1.08
2.26
8.02
Standard deviation
0.13
0.22
0.46
0.11
0.23
0.61
C V (%)
13.0
8.91
6.3
10.19
10.18
7.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MYO concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MYO in samples. No significant cross-reactivity or interference between Mouse MYO and analogues was observed.
