The Human Myo/Myoglobin CLIA Kit is specifically designed for the precise quantification of myoglobin levels in human biological samples such as serum and plasma. With its high sensitivity and specificity, this kit provides accurate and dependable results, making it suitable for various research applications.Myoglobin is a key protein that is found in skeletal and cardiac muscle tissues, serving as an important marker for muscle injury and related disorders. Elevated levels of myoglobin are commonly associated with conditions such as myocardial infarction and rhabdomyolysis, making it a valuable biomarker for monitoring and diagnosing these conditions.Overall, the Human Myo/Myoglobin CLIA Kit offers researchers a reliable tool for studying muscle-related disorders and exploring potential therapeutic interventions.
Product Name:
Human MYO (Myoglobin) CLIA Kit
SKU:
HUES00126
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
