The Myoglobin (MYO) ELISA Kit is specifically designed for the accurate quantitative measurement of myoglobin levels in human samples. Myoglobin, a heme protein found in skeletal and cardiac muscle, serves as an essential biomarker for detecting muscle injury and cardiac stress. Elevated levels of myoglobin in the blood are indicative of muscle damage, such as in cases of myocardial infarction or skeletal muscle trauma. This ELISA kit offers exceptional sensitivity and specificity, enabling precise and reproducible results in the quantification of myoglobin across various biological samples. Manufactured under strict quality control processes, the MYO ELISA Kit ensures reliable performance and user-friendly operation, making it suitable for both research and clinical applications. Trust in the MYO ELISA Kit from Assay Genie for precise and dependable analysis of this critical biomarker in your experimental or diagnostic studies.
Product Name:
Human MYO (Myoglobin) ELISA Kit
SKU:
AEES00060
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
