The Mouse OC-BGP Osteocalcin ELISA Kit is specifically designed for the precise measurement of levels of osteocalcin in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research purposes.Osteocalcin is a key protein involved in bone metabolism, playing a vital role in bone formation and mineralization. It is critical for maintaining bone health and is a valuable biomarker for studying conditions such as osteoporosis, bone fractures, and metabolic bone diseases. The Mouse OC-BGP Osteocalcin ELISA Kit provides researchers with a reliable tool for investigating the role of osteocalcin in these conditions and developing potential therapeutic interventions.
Product Name:
Mouse OC/BGP (Osteocalcin) ELISA Kit
SKU:
MOES01331
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
