The Human OC/BGP (Osteocalcin) ELISA Kit is specifically designed for the precise measurement of osteocalcin levels in human serum, plasma, and cell culture supernatants. This cutting-edge kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.Osteocalcin, also known as Bone Gla Protein (BGP), is a key protein involved in bone formation and regulation. It is essential for maintaining bone health and plays a crucial role in conditions such as osteoporosis, bone fractures, and other bone-related diseases. By measuring osteocalcin levels, researchers can gain valuable insights into bone metabolism and assess bone health status.With its advanced technology and rigorous validation process, the Human OC/BGP (Osteocalcin) ELISA Kit is a valuable tool for studying bone physiology, investigating bone disorders, and developing potential therapies for improving bone health. Trust in this reliable kit to deliver precise and accurate results for your research needs.
Product Name:
Human OC/BGP (Osteocalcin) ELISA Kit
SKU:
HUES02389
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.