The Mouse Stromal Cell-Derived Factor 1 (SDF-1/CXCL12) ELISA Kit is a cutting-edge tool meticulously developed to quantitatively measure SDF-1/CXCL12 levels in a wide range of biological samples with exceptional accuracy and precision. SDF-1, also known as CXCL12, is a crucial chemokine protein that plays a pivotal role in diverse cellular processes such as cell migration, survival, and angiogenesis. This versatile protein is a key regulator in various physiological and pathological conditions, including immune responses, stem cell mobilization, and cancer progression. By detecting and quantifying SDF-1/CXCL12 expression, researchers can gain valuable insights into its involvement in inflammatory responses, tissue repair mechanisms, and disease pathogenesis. Understanding the intricate functions of SDF-1/CXCL12 is essential for elucidating its impact on cellular behaviors and for exploring novel therapeutic strategies targeting this chemokine axis in different diseases. The Mouse SDF-1/CXCL12 ELISA Kit by Assay Genie offers outstanding sensitivity and specificity, enabling researchers to generate reliable and reproducible data for their investigations. Manufactured under rigorous quality control standards, this kit ensures robust performance and user-friendly protocols, making it an excellent choice for researchers conducting cutting-edge studies in the fields of immunology, oncology, and regenerative medicine. Partner with Assay Genie's SDF-1/CXCL12 ELISA Kit to uncover the intricate roles of SDF-1/CXCL12 in cellular processes and disease pathophysiology, paving the way for innovative research discoveries and therapeutic advancements.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
89-102
97-107
86-100
Average (%)
94
102
92
1:4
Range (%)
92-103
93-105
100-107
Average (%)
100
99
103
1:8
Range (%)
88-100
90-97
90-98
Average (%)
96
94
93
1:16
Range (%)
91-103
89-96
91-102
Average (%)
98
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
98-107
103
Cell Culture Media (n=8)
97-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
265.68
739.88
2403.84
248.87
761.08
2476.38
Standard deviation
17.27
47.8
165.14
16.67
59.36
197.62
C V (%)
6.5
6.46
6.87
6.7
7.8
7.98
Uniport ID:
P40224
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse SDF-1/CXCL12 in samples.No significant cross-reactivity or interference between Mouse SDF-1/CXCL12 and analogues was observed
