Human SDF-1 (Stromal Cell Derived Factor 1) ELISA Kit
The Human SDF-1 (Stromal Cell-Derived Factor-1) ELISA Kit is specifically designed to accurately detect levels of SDF-1 in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.SDF-1 is a critical chemokine involved in various biological processes, including stem cell homing, immune cell trafficking, and tissue repair. Its ability to attract stem cells and promote their migration to damaged tissues makes it a key player in regenerative medicine and tissue engineering. Additionally, SDF-1 has been implicated in diseases such as cancer, cardiovascular disorders, and inflammatory conditions, highlighting its importance as a biomarker for disease research and therapeutic development.Overall, the Human SDF-1 ELISA Kit provides a valuable tool for researchers seeking to study the role of SDF-1 in health and disease, enabling them to accurately measure its levels in biological samples and further explore its potential therapeutic applications.
Product Name:
Human SDF-1 (Stromal Cell Derived Factor 1) ELISA Kit
SKU:
HUES01339
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
84-96
86-99
95-111
Average (%)
90
91
103
1:4
Range (%)
95-109
89-103
92-106
Average (%)
103
94
100
1:8
Range (%)
95-110
81-94
93-107
Average (%)
102
87
100
1:16
Range (%)
96-108
83-93
93-108
Average (%)
102
88
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
88-101
93
Cell Culture Media (n=8)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.6
3.69
0.42
1.63
3.96
Standard deviation
0.02
0.11
0.15
0.03
0.1
0.34
C V (%)
5.3
6.9
4.2
7.7
6.32
8.7
Uniport ID:
P48061
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Human SDF-1/CXCL12 and analogues was observed
