Rat SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit
The Rat Stromal Cell-Derived Factor 1 (SDF-1/CXCL12) ELISA Kit is a specialized assay meticulously developed for the precise quantitative measurement of SDF-1 levels in various biological samples derived from rat models. SDF-1, also known as CXCL12, is a potent chemokine that plays a crucial role in cell migration, homing, and differentiation, exerting its functions by interacting with its cognate receptor CXCR4. This ELISA kit empowers researchers to accurately quantify SDF-1 concentrations, providing valuable insights into its involvement in diverse physiological and pathological processes such as stem cell mobilization, hematopoiesis, angiogenesis, and inflammation. By quantifying SDF-1 levels with high sensitivity and specificity, this kit enables researchers to elucidate the dynamic roles of SDF-1/CXCL12 in various biological systems. Manufactured under stringent quality control standards, the Rat SDF-1/CXCL12 ELISA Kit ensures robust performance and reproducible results, allowing researchers to explore the intricate role of SDF-1 in a wide array of cellular activities. With its exceptional sensitivity and reliability, this kit is an excellent choice for research studies focusing on the intricate mechanisms of cell migration, signaling, and tissue homeostasis in rat models.
Product Name:
Rat SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit
SKU:
AEES00541
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
