Human SDF-1 (Stromal Cell Derived Factor 1) CLIA Kit
The Human SDF-1 (Stromal Cell-Derived Factor 1) CLIA Kit is a highly efficient assay kit designed for the precise measurement of SDF-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.SDF-1, also known as CXCL12, is a critical chemokine involved in various biological processes, including stem cell homing, immune response modulation, and tissue repair. Dysregulation of SDF-1 has been linked to several diseases, including cancer, cardiovascular disorders, and inflammatory conditions, highlighting its importance as a potential diagnostic and therapeutic target. By utilizing the Human SDF-1 CLIA Kit, researchers can gain valuable insights into the role of SDF-1 in physiological and pathological processes, paving the way for the development of novel treatments and interventions. Trust in the accuracy and reliability of this kit to support your studies and advance our understanding of SDF-1 biology.
Product Name:
Human SDF-1 (Stromal Cell Derived Factor 1) CLIA Kit
SKU:
HUES00050
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.