The Rat SDF-1 Alpha (Stromal Cell-Derived Factor-1 Alpha) CLIA Kit is a powerful tool for precise measurement of SDF-1 Alpha levels in rat samples. This kit offers exceptional sensitivity and specificity, delivering dependable and consistent results for various research purposes.SDF-1 Alpha is a key chemokine involved in immune response and stem cell homing, playing a vital role in processes such as cell migration and tissue repair.
It is implicated in various diseases, including cancer, inflammatory disorders, and cardiovascular conditions, making it a valuable biomarker for investigating these pathologies and potential therapeutic interventions.Order the Rat SDF-1 Alpha CLIA Kit today and enhance your research with accurate and reliable measurements of SDF-1 Alpha levels in rat samples.
Rat SDF-1 alpha (Stromal Cell Derived Factor 1 alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SDF-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SDF-1α. You can calculate the concentration of Rat SDF-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
95-112
91-103
Average (%)
97
102
98
1:4
Range (%)
101-115
93-104
89-106
Average (%)
109
99
97
1:8
Range (%)
85-95
96-112
99-110
Average (%)
90
102
105
1:16
Range (%)
101-115
102-117
86-100
Average (%)
107
110
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
99
EDTA plasma (n=5)
84-100
91
Cell culture media (n=5)
94-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.44
57.84
230.7
21.51
54.78
213.08
Standard deviation
2.3
4.2
23.88
2.55
5.41
16.81
C V (%)
9.81
7.26
10.35
11.85
9.88
7.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SDF-1α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SDF-1α in samples. No significant cross-reactivity or interference between Rat SDF-1α and analogues was observed.
