Mouse sIgA (Secretory Immunoglobulin A) ColorStep ELISA Kit
The Mouse sIgA (Secretory Immunoglobulin A) ColorStep ELISA Kit from AssayGenie is a cutting-edge tool for precise measurement of sIgAColorStep levels in mouse serum, plasma, and other biological fluids. The kit delivers exceptional sensitivity and specificity, ensuring accurate quantification of sIgA across diverse sample types and concentration ranges. Secretory IgA is the predominant immunoglobulin at mucosal surfaces and functions as a critical first-line defense in the innate immune response, neutralizing pathogens and toxins in the respiratory, gastrointestinal, and urogenital tracts. sIgA dysregulation is associated with increased susceptibility to mucosal infections, inflammatory bowel disease, and defective barrier function in allergic and autoimmune conditions.
Equipped with this ELISA kit, researchers can delve deep into the intricate mechanisms governed by sIgAColorStep and gain valuable insights into its involvement in different biological pathways. The AssayGenie Mouse sIgA (Secretory Immunoglobulin A) ColorStep ELISA Kit provides robust quantification to support rigorous investigation of mucosal immunity, vaccine response, and immune homeostasis in translational mouse models.
Product Name:
Mouse sIgA (Secretory Immunoglobulin A) ColorStep ELISA Kit
SKU:
AEFI03972
Reactivity:
Mouse
Detection Method:
Sandwich ELISA
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, And Other Biological Fluids
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-105%
83-101%
82-98%
EDTA Plasma (n = 5)
85-104%
84-101%
80-96%
Heparin Plasma (n = 5)
88-95%
82-95%
83-93%
Recovery:
Matrix
Recovery Range (%)
Average (%)
Serum (n = 5)
93-101
97
EDTA Plasma (n = 5)
93-102
97
Heparin Plasma (n = 5)
88-101
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate to room temperature for at least 30 minutes. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Standard, Sample & Control Loading: Aliquot 100 µl of standard solutions into the standard wells, 100 µl of properly diluted sample into test sample wells, and 100 µl of Sample Dilution Buffer into the control (blank) well.
3
First Incubation & Wash: Seal the plate and incubate at 37°C for 90 minutes. Aspirate the liquid and wash the plate 2 times with Wash Buffer.
4
Biotin-labelled Antibody & Wash: Add Biotin-labelled Antibody working solution to each well and incubate at 37°C for 60 minutes. Wash the plate 3 times, allowing the buffer to stay in the wells for 1-2 minutes per wash. Add SABC working solution and incubate at 37°C for 30 minutes, then wash 5 times.
5
Color Development: Add 90 µl of TMB substrate into each well and incubate at 37°C in the dark for 10-20 minutes.
6
Stop Reaction & Reading: Add 50 µl of Stop Solution and measure absorbance at 450 nm in a microplate reader immediately.
Sample Type
Protocol
Serum
Allow whole blood to coagulate at room temperature (2 h) or 2-8°C overnight. Centrifuge at 1000 × g for 20 min and collect the supernatant. Store or use immediately.
Plasma
Collect in anticoagulant tubes (EDTA, citrate, or heparin), mix gently, and centrifuge within 30 min at 1000 × g, 2-8°C for 15 min. Store or assay as needed.
Tissue Homogenate
Homogenize tissue in 50 mM Tris + 0.9% NaCl + 0.1% SDS (pH 7.3) with protease inhibitors (e.g. 1 mM PMSF). Avoid NP-40, Triton X-100 and DTT. Disrupt by ultrasonic homogenization or freeze-thaw cycles, centrifuge at 5000 × g for 5 min and collect the supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes at 2-8°C and collect the clarified cell culture supernatant.
Cell Lysate
Lyse cells in lysis buffer with protease inhibitor (e.g. 1 mM PMSF) on ice for 30-60 minutes, or by ultrasonic treatment. Centrifuge at 10,000 rpm for 10 min at 2-8°C and collect the supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
