The Human SIGA (Secretory Immunoglobulin A) ELISA Kit is a cutting-edge tool designed for the accurate measurement of SIGA levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for various research applications.SIGA is an important component of the immune system, particularly in mucosal immunity, providing defense against pathogens in the mucous membranes of the respiratory, gastrointestinal, and genitourinary tracts. Monitoring SIGA levels can offer valuable insights into the body's immune response and help researchers better understand conditions related to mucosal immunity, such as infections and autoimmune disorders.Overall, the Human SIGA ELISA Kit is a valuable resource for researchers studying immune responses, mucosal immunity, and infectious diseases, offering reliable data to advance scientific understanding and potentially lead to the development of novel therapeutic interventions.
Product Name:
Human sIgA (Secretory Immunoglobulin A) ELISA Kit
SKU:
HUES02333
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
