The Mouse TPO (Thrombopoietin) ELISA Kit is specifically designed for the accurate detection of thrombopoietin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Thrombopoietin is a key regulator of platelet production and a critical factor in maintaining normal blood clotting functions. Dysregulation of thrombopoietin levels can lead to disorders such as thrombocytopenia and myeloproliferative neoplasms, making it an important biomarker for studying these conditions and developing potential treatments.With this Mouse TPO ELISA Kit, researchers can confidently measure thrombopoietin levels in mouse samples with accuracy and precision, advancing their understanding of hematopoiesis and related diseases.
Product Name:
Mouse TPO (Thrombopoietin) ELISA Kit
SKU:
MOES01147
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
