The Human TPO (Thrombopoietin) ELISA Kit is specifically designed for the accurate measurement of TPO levels in human serum, plasma, and cell culture supernatants. This kit offers a high level of sensitivity and specificity, ensuring the reliability and reproducibility of results, which makes it ideal for various research applications in the field.TPO is a key regulator of platelet production and plays a crucial role in maintaining normal levels of platelets in the blood. Imbalances in TPO levels have been associated with thrombocytopenia, myelodysplastic syndromes, and some cases of immune thrombocytopenia. Therefore, the detection of TPO levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Human TPO ELISA Kit is a valuable tool for researchers studying platelet disorders, hematopoiesis, and related diseases, offering accurate and reliable measurements of TPO levels for further investigation and analysis.
Product Name:
Human TPO (Thrombopoietin) ELISA Kit
SKU:
HUES02598
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
