The Mouse TSH (Thyroid Stimulating Hormone) ELISA Kit is specifically designed for the precise measurement of TSH levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring dependable and consistent results, which makes it perfect for a variety of research purposes.Thyroid Stimulating Hormone is a key hormone that regulates the thyroid gland's function, controlling the production of thyroid hormones that affect metabolism, growth, and other essential bodily functions. Dysregulation of TSH levels can indicate thyroid dysfunction, making it a valuable biomarker for studying thyroid-related conditions and potential treatment strategies.Overall, the Mouse TSH ELISA Kit offers researchers a reliable tool to accurately quantify TSH levels in mouse samples, aiding in the advancement of research in areas such as endocrinology, metabolism, and thyroid disorders.
Product Name:
Mouse TSH (Thyroid Stimulating Hormone) ELISA Kit
SKU:
MOES01557
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
