The Rat TSH (Thyroid Stimulating Hormone) ELISA Kit is a highly reliable and sensitive kit designed for the accurate measurement of TSH levels in rat serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it suitable for a variety of research applications.TSH is a key hormone in the regulation of the thyroid gland, playing a crucial role in controlling metabolism and energy levels in the body. Imbalances in TSH levels can indicate thyroid dysfunction, making it a valuable biomarker for studying thyroid disorders and related conditions.By using the Rat TSH ELISA Kit, researchers can accurately quantify TSH levels in rat samples, allowing for a better understanding of thyroid function and the development of potential treatments for thyroid-related diseases.
Product Name:
Rat TSH (Thyroid Stimulating Hormone) ELISA Kit
SKU:
RTES00816
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
