Mouse VEGF-C (Vascular Endothelial cell Growth Factor C) CLIA Kit
The Mouse VEGF-C (Vascular Endothelial Cell Growth Factor-C) CLIA Kit is a highly sensitive and specific assay designed for the quantification of VEGF-C levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.VEGF-C is a key player in the regulation of lymphangiogenesis and angiogenesis, playing a vital role in various physiological and pathological processes.
By accurately measuring VEGF-C levels, researchers can gain valuable insights into the mechanisms underlying diseases such as cancer, inflammation, and lymphatic disorders, paving the way for the development of potential therapeutic interventions.Overall, the Mouse VEGF-C CLIA Kit offers a powerful tool for studying the role of VEGF-C in disease pathogenesis and for exploring new therapeutic strategies targeting this important growth factor.
Product Name:
Mouse VEGF-C (Vascular Endothelial cell Growth Factor C) CLIA Kit
SKU:
MOES00590
Target:
Mouse VEGF-C (Vascular Endothelial cell Growth Factor C)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse VEGF-C. You can calculate the concentration of Mouse VEGF-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
101-115
95-113
Average (%)
109
107
103
1:4
Range (%)
99-116
91-105
103-120
Average (%)
107
96
109
1:8
Range (%)
85-97
93-106
94-109
Average (%)
90
99
101
1:16
Range (%)
101-114
97-114
95-107
Average (%)
107
104
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
89-104
97
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.37
120.9
399.97
37.99
117.06
400.99
Standard deviation
4.65
13.79
27.52
4.11
12.03
33.2
C V (%)
11.81
11.41
6.88
10.82
10.28
8.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse VEGF-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VEGF-C in samples. No significant cross-reactivity or interference between Mouse VEGF-C and analogues was observed.
