The Mouse VWF (Von Willebrand Factor) CLIA Kit is specifically designed for the accurate measurement of Von Willebrand Factor levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research studies.Von Willebrand Factor is a key protein involved in blood clotting and platelet function, playing a critical role in hemostasis and thrombosis.
Dysregulation of VWF levels has been implicated in various bleeding disorders and cardiovascular diseases, making it a valuable biomarker for investigating these conditions and potential treatment options.With this Mouse VWF CLIA Kit, researchers can confidently analyze VWF levels in mouse samples, providing valuable insights into the mechanisms underlying hemostasis and thrombosis, as well as potential therapeutic targets for related diseases.
Product Name:
Mouse vWF (von Willebrand Factor) CLIA Kit
SKU:
MOES00596
Target:
Mouse vWF (von Willebrand Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse vWF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse vWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse vWF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse vWF. You can calculate the concentration of Mouse vWF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
91-104
92-102
Average (%)
96
96
97
1:4
Range (%)
93-107
90-101
92-105
Average (%)
100
96
98
1:8
Range (%)
88-99
101-116
84-96
Average (%)
93
107
90
1:16
Range (%)
88-102
94-107
89-103
Average (%)
94
99
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
97
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
2.29
9.1
0.94
2.27
9.14
Standard deviation
0.1
0.21
1.0
0.12
0.23
0.64
C V (%)
10.42
9.17
10.99
12.77
10.13
7.0
Sample type &Sample volume:
Plasma; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse vWF concentrations in Plasma.
Specificity:
This kit recognizes Mouse vWF in samples. No significant cross-reactivity or interference between Mouse vWF and analogues was observed.
