MPO Activity Assay Kit
MYELOPEROXIDASE (MPO; EC 220.127.116.11) is a peroxidase enzyme and can be found in neutrophil, monocytes, and some soft tissue macrophages. MPO has an ability to use chloride as a cosubstrate with hydrogen peroxide to generate hypochlorous acid, a powerful antimicrobial agent produced by neutrophils. However, an excessive production of hypochlorous acid can lead to oxidative stress and tissues damage. Inflammation may also result when MPO oxidizes various substances such as phenols and anilines. Studies show that increased MPO levels may increase the risk of myocardial infarction and cardiovascular disease.
Assay Genie' s myeloperoxidase (MPO) assay kit is based on the MPO enzyme reaction with hydrogen peroxide (H2O2) which oxidizes the dye reagent to a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the total peroxidation activity in the sample. The provided MPO inhibitor is used to suppress peroxidase activity due to MPO in order to differentiate other peroxidase activities that may be present in the samples.
MPO peroxidation activity determination in biological samples (e.g. cell lysates, tissues, etc.)
- Fast and sensitive. Linear detection range (20 uL sample): 0.0025 to 2 U/L for 10 min reaction at 25°C.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
MPO Activity Assay Kit Information:
|Kit Includes:||Assay Buffer: 15 mL, Resorufin: 1.5 mL, Dye Reagent: 120 uL, MPO Inhibitor: 1.5 mL, 3% Stabilized H2O2: 100 uL|
|Kit Requires:||Pipetting devices, centrifuge tubes, black 96-well plates and plate reader capable of measuring fluorescence at lex/em= 530/585 nm|
|Method of Detection:||FL530/585nm|
|Detection Limit:||0.0025 U/L|
|Samples:||Cell lysates, tissues, etc|
|Protocol Length:||Approximately 30 min|
|Storage:||Store all components at -20°C upon receiving|
|Shelf Life:||6 months|