Description
Monkeypox Virus (MPXV) A33R Protein IgG ELISA Kit
The Monkeypox Virus A33R Protein Human IgG ELISA Kit is a highly specific and sensitive assay designed for the precise detection of human IgG antibodies against the A33R protein of the Monkeypox virus. This kit provides reliable and reproducible results, making it ideal for research applications aimed at studying the immune response to Monkeypox virus infection.The A33R protein is a key antigen of the Monkeypox virus, which belongs to the same family as smallpox and causes a similar but less severe disease in humans.
Detection of IgG antibodies against the A33R protein can help in the diagnosis of Monkeypox virus infection and monitoring of immune response post-infection.This ELISA kit is easy to use and provides accurate results within a short period of time, thus facilitating research efforts in understanding the pathogenesis of Monkeypox virus infection and the development of potential treatments and vaccines.
| Product Name: | Monkeypox Virus (MPXV) A33R Protein IgG ELISA Kit |
| Product Code: | MKRS0009 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
