Description
Monkeypox Virus (MPXV) B16R Protein IgA ELISA Kit
The Monkeypox virus B16R Protein Human IgA ELISA Kit is a cutting-edge diagnostic tool designed for the accurate detection of IgA antibodies specific to the B16R protein of the Monkeypox virus in human serum, plasma, and cell culture supernatants. This innovative kit features high sensitivity and specificity, guaranteeing precise and consistent results for a variety of research applications.The B16R protein is a key component of the Monkeypox virus, playing a crucial role in the viral replication process and host immune response.
Detection of IgA antibodies against the B16R protein can provide valuable insights into the immune response to Monkeypox virus infection, aiding in the diagnosis and monitoring of this infectious disease.Overall, the Monkeypox virus B16R Protein Human IgA ELISA Kit is an essential tool for researchers and healthcare professionals interested in studying Monkeypox virus infection, immune response dynamics, and potential therapeutic interventions.
| Product Name: | Monkeypox Virus (MPXV) B16R Protein IgA ELISA Kit |
| Product Code: | MKRS0014 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
