Description
Monkeypox Virus (MPXV) B16R Protein IgG ELISA Kit
The Monkeypox Virus B16R Protein Human IgG ELISA Kit is a reliable and accurate tool for the detection of monkeypox virus B16R protein-specific human IgG antibodies in biological samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Monkeypox virus is a zoonotic virus that can cause a smallpox-like illness in humans, with symptoms including fever, headache, muscle aches, and rash. The B16R protein is a key target for immune response against the virus, making it a valuable marker for monitoring immune responses and vaccine efficacy.
By detecting and quantifying B16R-specific IgG antibodies, researchers can gain valuable insights into immune responses to monkeypox virus infection and vaccination, aiding in the development of effective diagnostics and therapeutics. The Monkeypox Virus B16R Protein Human IgG ELISA Kit is essential for studying the pathogenesis of monkeypox virus and evaluating vaccine effectiveness in both preclinical and clinical research settings.
| Product Name: | Monkeypox Virus (MPXV) B16R Protein IgG ELISA Kit |
| Product Code: | MKRS0015 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
